REGENERATION POTENTIALITY OF POTATO IN IN VITRO CONDITION UNDER DIFFERENT HORMONAL COMBINATIONS
The present investigation was conducted to study the effects of different concentrations and combinations of growth regulators on the callus induction and plant regeneration of potato cvs. Diamant, Cardinal and Granula.The internodal segments of three virus tested potato cultivars were used for the establishment of culture.The interaction effects between cultivars and growth regulators showed significant differences for all the parameters used in the experiment except only root length. The in vitro callus proliferation was the best in Diamant when MS medium was fortified with 3.0 mgL-1 2,4-D + 0.25 mgL-1KIN in terms of the highest percentage (100) of callus, the maximum callus size (11.04 mm), the maximum fresh weight (1.126 g) of callus and the maximum dry weight (0.1044 g) of callus. In case of shoot regeneration, MS medium fortified with 1.0 mgL-1BAP+ 0.5 mgL-1GA3 in Granula was best in terms of the highest no. of shoots/callus (2.920), no. of nodes/shoot (6.50) and shoot length (6.760 cm) with the minimum days (14.40). The root regeneration showed the highest results in terms of no. of roots/plantlets(10.80) in Cardinal when MS medium was fortified with 1.0 mgL-1IAA + 0.25 mgL-1GA3. It was found that the in vitro regeneration and multiplication potentiality was the highest in the variety Granula followed by Cardinal and Diamant.
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