ASSESSMENT OF GENETIC DIVERSITY AMONG SIXTY FIVE BRINJAL GENOTYPES USING SSR MARKERS

Abstract

The present study was conducted at the Biotechnology Laboratory and Horticulture Field Laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA) to study the genetic diversity among 65 brinjal genotypes using single sequence repeats (SSR) markers. Molecular characterization of the collected genotypes was performed using six SSR markers. The selected primers, namely EM107, EM114, EM133, EM139, EM140 and EM145 showed polymorphism. The values of pair-wise comparison of Nei’s genetic distance (D) between genotypes computed from combined data for the 6 primers ranged from 0.17 to 1.00. The highest genetic dissimilarity (100%) was observed among various combinations, such as Islampuri (S31), Thapara (S32), Mentar (S33), Iribegun (S35), Eye-red (S36), Deembegun (S37), Comilla-L (S38) and Chega (S39) with Dohazari G (S10), Borka (S11), Khatkhatia B (S12), Khatkhatia BAU (S13), ISD-006 (S2) and Laffa-M (S3). The UPGMA cluster based on Nei’s genetic distance analysis led to the grouping of the 65 brinjal genotypes into three major clusters with nine sub-clusters.

References

Adeniji, O.T., Kusolwa, P., Reuben, S.O.W.M. and Deo, P.2012. Molecular diversity among seven Solanum (eggplant and relatives) species assessed by the simple sequence repeats (SSRs) markers. African J. Biotechnol., 11(90): 15643-15653.

Bahera, T.K., Sharma, P., Singh, B.K., Kumar, R., Mohapotra, T. and Singh, N.K. 2006. Assessment of genetic diversity and species relationship in eggplant (S. melongene L) using STMS markers Sci. Hort. 107:352-357.

BBS-2019. Monthly Statistical Bulletin (May). 2020. Bangladesh Bureau of Statistics. Statistics Division, Ministry of Planning, Government of the People’s Republic of Bangladesh. p. 43.

Demir, K, M. Bakir G. Sarikamiş and S. Acunalp. 2010. Genetic diversity of eggplant (Solanum melongena) germplasm from Turkey assessed by SSR and RAPD markers. Genet. Mol. Res., 10; 9(3):1568-1576.

Dunlop, F. 2006. Revolutionary Chinese cook book. Recipes from Hunan Province. Ebury Pess. 202p.

FAO. 2020. FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy (http://www.fao.org/faostat/en/#home).

Isshiki S., Iwata, N.and Khan, M.R. 2008. ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Sci. Hort., 117: 186-190.

Kalloo, G. 1993. Eggplant (Solanum melongena L.). In: Genetic Improvement of Vegetable Crop (Kalloo, G. and B.O. Bergh, eds.). Pergamon Press, Oxford, 587-604.

Khorsheduzzaman, A.K.M., Alam, M.Z., Rahman, M.M., Mian, M.A.K. and Hossain, M.M. 2008. Molecular characterization of five selected brinjal (Solanum melongena L.) genotypes using SSR markers. Bangladesh J. Plant Breed. Genet., 21(1): 01-06.

Kumar, V.A., Nagangoud, A. and Patil, B.V. 2003. Biology of brinjal shoot and fruit borer, Leucinodes orbonalis Guenee. Pest Management in Horticultural Ecosystems, 9(2): 127-131.

Liu, K. and Muse, S.V. 2005. Power Marker: Integrated Analysis Environment for Genetic Marker Data. Bioinformatics, 21, 2128-2129. (http://dx.doi.org/10.1093/bioinformatics/ bti282)

Mace, E.S., Lester, R.N. and Gebhardt, C.G. 1999. AFLP analysis of genetic relationships among the cultivated eggplant, Solanum melongena L., and wild relatives. Theor. Appl. Genet., 99: 626-633.

Nei, M. 1973. Analysis of gene diversity in subdivided populations. In: Proceedings of National Academy of Science, USA, 70:3321-3323.

Nunome, T.S. Negoro, I. Kono and H. Kanamori. 2009. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl. Genet., 119: 1143-1153.

Nunome, T., Ishiguro, K., Yoshida, T. and Hirai, M. 2001. Mapping of fruit shape and color development traits in eggplant (Solanum melongena L.) based on RAPD and AFLP markers. Breed. Sci., 51: 19-26.

Nunome, T., Suwabe, K., Iketani, H. and Hirai, M. 2003. Identification and characterization of microsatellites in eggplant. Plan Breed., 122: 256-262.

Rohlf, F.J. 2000. NTSYS pc2.1: numerical taxonomy and multivariate analysis system version 2.1. Applied Biostatistics Inc New York.

Shaukat, M.A., Ahmad, A. and Mustafa, F. 2009. Evaluation of resistance in brinjal (Solanum melongena L.) against brinjal shoot and fruit borer (Leucinodes orbonalis Guen.) infestation: A Review. Intl. J. Appl. Sci. Biotechnol., 6(3): 199-206.

Stagel, A., Portis, E., Toppino, L. and Rotino, G.L. 2008. Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC Genomics,         9: 357.