STUDY ON EFFICACY OF TRICHODERMA IN BIOLOGICAL CONTROL AGAINST PURPLE BLOTCH OF ONION

  • M.K. Hasna Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202, Bangladesh

Abstract

The efficacy of the antagonistic microorganism, Trichoderma harzianum in controlling purple blotch of onion (Alternaria porri) was investigated in the laboratory and field of Plant Pathology Division of the Bangladesh Institute of Nuclear Agriculture, Mymensingh. The antagonistic activity of three isolates of T. harzianum was observed against A. porri through dual culture technique and the superior one was used in the field experiment. Five treatments were given viz. T1: Seed treatment with T. harzianum, T2: Seedling treatment with T. harzianum, T3: Foliar application with T. harzianum, T4: Seed treatment and seedling treatment with T. harzianum and T5: Control (A. porri in absence of T. harzianum). The variety BARIpiaj-1 was used as tested crop. All treatments significantly reduced purple blotch disease intensity compared to the control. The least disease intensity on leaf and flower stalk was found in the treatment T4 (Seed and seedling treatment with T. harzianum) followed by the treatment T1 (Seed treatment with T. harzianum). The highest disease reduction over control (63.1% on leaf and 47.18% on flower stalk) was observed in the treatment T4 (seed and seedling treatment with T. harzianum) followed by the seed treatment only (T1) which gave disease reduction 58.7% on leaf and 37.03% on flower stalk over control. Therefore, application of T. harzianum both in seed and seedling was more effective in reducing disease intensity than individual application of T harzianum in seed or seedling.

References

Abdel-Hafez, S.I., Abo-Elyousr, K.M. and Abdel Rahim, R.I. 2014. Effectiveness of plant extracts to control purple blotch and stemphylium diseases of onion (Allium cepa L.) in Assiut, Egypt. Arch. Phytopathol. Plant Prot. 47 (3): 377-387.

Altintas, S. and Bal, U. 2008. Effect of the commercial product based on Trichoderma harzianum on plant, bulb and yield characteristics of onion. Sci. Hortic, 116: 219-222.

Amin, F, Razdan, V.K., Mohiddin, F.A, Bhat, K.A. and Banday, S. 2010. Potential of Trichoderma species as biological agent of soil borne fungal propagules. J. Phytopath. 2: 38-41.

BBS, 2020. Statistical Year Book of Bangladesh. Bangladesh Bureau of Statistics, Statistics Division, Ministry of Planning. Government of People̕s Republic of Bangladesh.

Begum, M., Hossain, M.I. and Hoque, M.S. 1998. Biocontrol of seed borne Fusrium oxysporum with Trichoderma harzianum. Bangladesh J. Environ. Sci. 4: 128-133.

Chethana, B., Ganeshan, G., Rao, A.S. and Bellishree, K. 2013. In vitro evaluation of plant extracts, bioagents and fungicides against Alternaria porri (Ellis) Cif, causing purple blotch disease of onion. Pest Manage. Hortic. Ecosystem. 18: 194-198.

Dubey, S.C. and Patel, B. 2002. Mass multiplication of antagonists and standardization of effective dose for management of web blight of urd and mung bean. Indian Phytopath. 55(3): 338-341.

Elad, Y. 2000.  Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection. 19: 709-714.

Gajera, H., Domadiya, R., Patel S., Kapopara, M. and Golakiya, B. 2013. Molecular mechanism of Trichoderma as biocontrol agents against phytopathogens system- a review. Current Research in Microbiology and Biotechnology. 1(4):133-142.

Harman, G.E. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology. 96: 190-194.

Howell, C. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis. 87: 4-10.

Imtiaz, A. and Lee, T. 2008. Antagonistic effect of three Trichoderma species on Alternaria porri pathogen of onion blotch. World J. Agril. Sci. 4: 13-17.

 Islam,  M.M., Begum, F.,  Nahar, N.,  Habiba, U.A. and  Fakruzzaman, K.M. 2020. In-vivo management of purple blotch of onion caused by Alternaria porri (Ellis) Cif. through fungicides. American J. Plant Sci. 11(11): 1847-1859.

Kumar, S. 2013. Trichoderma: A biological weapon for managing plant diseases and promoting sustainability. Indian J. Agril. Sci.Vet. Med. 1(3): 106-121.

Meah, M.B. and Khan, A.A. 1987. Check list of fruit and vegetable diseases in Bangladesh. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh. p. 23.

Mishra, S. 2019. Disease control potential of Trichoderma harzianum and Trichoderma viride against purple blotch of onion. J. Pharmacognosy and Phytochemistry. 8(5): 368-371.

Mimbs IV, W.H., Cusaac, J.P.W., Smith, L.M., McMurry, S.T. and Belden, J.B. 2016. Occurrence of current-use fungicides and bifenthrin in rainwater basin wetlands. Chemosphere. 159:275-81.

Prakasam, V. and Sharma, P. 2012. Trichoderma harzianum (Th-3) a potential strain to manage the purple blotch disease of onion (Allium cepa L.) caused by Alternaria porri under North Indian plains. J. Agril. Sci. 4 (10): 266-272.

Rahim, M.A. 1991. Onion situation in Bangladesh: Problems, Research and future prospects. Allium Improvement Newsletter (USA), 1:1-10.

Rahman, M.I. 1990. Efficacy of fungicides in controlling purple leaf blotch (Alternaria porri) of onion (Allium cepa L.). MS Thesis. Department of Plant Pathology. Bangladesh Agricultural University. Mymensingh. p. 34.

Rini, C.R. and Sulochana, K.K. 2007. Usefulness of Trichoderma and Pseudomonas against Rhizoctonia solani and Fusarium oxysporum infecting tomato. J. Trop. Agric. 45:2-8.

Shahnaz, E., Razdan, V., Rezwi, S., Rather, T., Gupta, S. and Andrabi, M. 2012. Integrated disease management of foliar blight disease of onion: a case study of application of confounded factorials. J. Agril. Sci. 5: 17-22.

Sharma, S.R. 1986. Effect of fungicidal sprays on purple blotch and bulb yield of onion. Indian Phytopathol. 39:78-82.

Tuite, J. 1969. Plant pathological methods: fungi and bacteria.  Burgess Publ. Co. Minneapolis, Minnesota.

Tyagi, S., Dubey, V.P. and Charya, M.U. 1990. Biological control of purple blotch of onion caused by A. porri (Ellis.) ciferri, Tropical Pest Management. 36: 384-386.

Verma, L.R. and Sharma, R.C. 1999. Diseases of Horticultural Crops, Vegetables, Ornamentals and Mushrooms. Indian Publishing Co., New Delhi.

Wheeler, B.E. 1969. An Introduction to Plant Diseases. John Wiley and Sons Ltd., London, UK.

Section
Research Article