• S. Tasmin Horticulture division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202

    M.R. Islam Horticulture division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202

    M.N.H. Mehedi Horticulture division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202

    K.T. Akter Horticulture division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202

    M.A. Tarafder Soil Science Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202


Mutagenesis using ionizing radiation has been widely used for the genetic variability with novel characteristics. To know the optimum dose in black cumin an experiment was carried out at Bangladesh Institute of Nuclear Agriculture, HQs farm, Mymensingh. Collected three germplasm (BC10861, BC10863 and BC10864) of black cumin seeds were irradiated with different doses of gamma radiation as 50 Gy, 100 Gy, 150 Gy, 200 Gy, 250 Gy and 300 Gy to determine the LD50 for development of desirable mutants. Maximum seed germination (94.5-97.1%) was found from the lowest dose (50 Gy) of gamma radiation, while the minimum germination (18.2-22.0%) was obtained from the highest dose (300 Gy) among the three germplasm BC10861, BC10863 and BC10864. The seedlings survivability was 45-49% at 150 Gy. After one month, in the later growth stage none of the seedlings survived at the dose of 250 Gy and 300 Gy. At lower dose of 50 Gy maximum numbers of leaves (6.12-7), number of roots per plant (7.13-8.05), root length (5.34-7.1 cm), shoots length (5.3-7.1 cm) and fresh weight per plant (0.25-0.29 g) were observed among the germplasm. The seed germination and seedlings survival decreased with the radiation dose increased. The seedling growth parameters like number of leaves, root length, shoot length and fresh weight per plant reduced around 50% at 150 Gy dose of gamma radiation. Among the six doses of irradiation the LD50 was close to 150 Gy which caused around 55-51% death of seedlings, 45-49% seedlings survival and around 50% reduction in plant growth. Hence, it is concluded that 150 Gy may be the optimum dose for creating useful mutation induction in black cumin.


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