PROTOCOL DEVELOPMENT OF IRRADIATED RICE CALLI AND SUBSEQUENT IN VITRO REGENERATION (Short communication)

Abstract

Fine and aromatic rice varieties, such as Kataribhog, hold significant commercial value in Bangladesh, particularly in region like Dinajpur where Kataribhog is primarily cultivated. Despite its popularity, Kataribhog rice has a low yield (less than 2.50 t ha-1) and is prone to lodging. To address these issues, a study was conducted using in vitro culture techniques combined with gamma irradiation (60Co) to improve the cultivar. Three different media (PT-100G, PT-011G, and PT-100) were used to induce callus, followed by exposure to varying radiation doses (10Gy, 12Gy, and 15Gy). The PT-100G medium found the most effective, inducing an embryogenic callus formation rate of 32.93%, while PT-011G and PT-100 showed lower rates 27.87% and 11.11%, respectively. A radiation dose of 10Gy was found optimal, with an 80% survival rate for calli and the best plant regeneration outcomes.

References

Alam, M.A., Rahman, M.S. and Hossain, M.A. 2021. The effect of gamma radiation on in vitro regeneration and rooting in rice (Oryza sativa L.). Plant Biotechnology Reports, 15(1): 34-42.

Aditya, T.L., Hoque, M.E. and Khalequzzaman, M. 2004. Response to high frequency callus induction ability from root regions of germinated embryo in indica rice. Pakistan  Journal of  Biological Sciences, 7(5): 861-864.

Azria, D. and Bhalla, P.L. 2000. Plant regeneration from mature embryo-derived callus of Australian rice (Oryza sativa L.) varieties. Australian Journal of Agricultural Research, 51(2): 305-312.

BBS (Bangladesh Bureau of Statistics) 2020. Yearbook of agricultural statistics of Bangladesh. Government of Bangladesh, Dhaka.

Bouis, H.E. and Welch, R.M. (2010). Biofortification-a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop science, 50(1): 5-20.

Briside, E.A., Taniguchi, T. and Maeda, E. 1990. In vitro plant regeneration from morphogenic callus cultures of cultigens and wild Oryza species. Japan Journal of Crop Science, 59(3): 557- 565.

Dai, X.J., Yang, Y.Z., Zhou, L, Ou, L.J., Liang, M.Z., Li, W.J. and Chen, L.B. 2012. Analysis of indica-and japonica-specific markers of Oryza sativa and their applications. Plant Systematics and Evolution., 298(2): 287-296.

FAO, 2021. FAOSTAT - Food and Agriculture Data. Food and Agriculture Organization of the United Nations.

Gao, Z.Y. and Huang, D.N. 1999. Some factors influencing callus formation and plant regeneration in indica rice varieties. Plant Physiology Communications, 35(2): 113-115.

Hossain, M.S. and Majumder, A.K. 2018. Impact of climate change on agricultural production and food security: a review on coastal regions of Bangladesh. International Journal of Agricultural Research, Innovation and Technology (IJARIT), 8(2355-2020-1646): 62-69.

Internationl Rice Research Institute (IRRI), 2003. Looking up in Laos. Rice Today, April, 6.

Katiyar, S.K., Chandel, G., Singh, P. and Pratibha, R. (1999). Genetic variation and effect of 2, 4-D on in-vitro plant regeneration in indica rice cultivars. Oryza, 36(3): 254-256.

Khan, S., Bhatti, M. I. and Arif, A. 2019. Influence of gamma irradiation on rooting and regeneration in crop plants: A review. Advances in Agricultural Science, 7(2): 101-115.

Khush, G.S. 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Molecular Biology, 59(1): 1-6.

Majeed, A., Muhammad, Z. and Anjum, S. 2018. Effects of gamma irradiation on plant tissue culture and its implications in crop improvement. Plant Science Today, 5(1): 45-52.

Rahman, M. A., Hasanuzzaman, M. and Jahan, N. 2020. Impact of gamma irradiation on callus induction and plant regeneration in rice (Oryza sativa L.). Journal of Plant Research and Biotechnology, 14(2): 10-18.

Seraj, Z.I., Islam, Z., Faroque, M.O., Devi, T. and Ahmed, S. 1997. Identification of the regeneration potential of embryo derived calluses from various indica rice varieties. Plant Cell Tissue and Organ Culture, 48: 9-13.

Tyagi, A.K., Khurana, J.P., Khurana, P., Raghuvanshi, S., Gaur, A., Kapur, A. and Sharma, S. 2004. Structural and functional analysis of rice genome. Journal of Genetics, 83(1): 79-99.

Wang, Y. and Li, J. 2005. The plant architecture of rice (Oryza sativa). Plant Molecular Biology, 59(1): 75-84.

Zhenyu, G. and Danian, H. 1999. Some factors influencing the callus formation and plant regeneration in indica rice varieties. Zhiwu Shenglixue Tongxun (China).