ASSESSMENT OF GENETIC DIVERSITY AMONG SIXTY FIVE BRINJAL GENOTYPES USING SSR MARKERS
The present study was conducted at the Biotechnology Laboratory and Horticulture Field Laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA) to study the genetic diversity among 65 brinjal genotypes using single sequence repeats (SSR) markers. Molecular characterization of the collected genotypes was performed using six SSR markers. The selected primers, namely EM107, EM114, EM133, EM139, EM140 and EM145 showed polymorphism. The values of pair-wise comparison of Nei’s genetic distance (D) between genotypes computed from combined data for the 6 primers ranged from 0.17 to 1.00. The highest genetic dissimilarity (100%) was observed among various combinations, such as Islampuri (S31), Thapara (S32), Mentar (S33), Iribegun (S35), Eye-red (S36), Deembegun (S37), Comilla-L (S38) and Chega (S39) with Dohazari G (S10), Borka (S11), Khatkhatia B (S12), Khatkhatia BAU (S13), ISD-006 (S2) and Laffa-M (S3). The UPGMA cluster based on Nei’s genetic distance analysis led to the grouping of the 65 brinjal genotypes into three major clusters with nine sub-clusters.
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