IN VITRO REGENERATION POTENTIALITY OF STRAWBERRY FROM DIFFERENT EXPLANT UNDER VARIOUS HORMONAL CONCENTRATIONS
An experiment was carried out to examine the effects of explants and different combinations of plant growth regulators in vitro micro propagation of strawberry. The research was laid out in 2 factorial experiments in completely randomized design. For shoot induction, six concentrations viz. BAP (0.5, 1.0, 1.5), GA3 (0.5) and KIN (0.1, 0.5) and for root induction four combinations viz. IBA (0.1, 0.5) and 2, 4-D (0.1, 0.5) were used. The interaction effects between explants and growth regulators showed significant differences for all the parameters used in the experiment. In case of shoot initiation, runner tip explants performed the best for days required to shoot initiation (8.00), number of shoot per plantlet (11.20), number of leaves per plantlet (12.80) and shoot length (4.70 cm) when 1.0 mg BAP + 0.5 mg GA3 + 0.5 mg KIN/L was used. In case of root initiation, runner tip explants exhibited the best results for days to root initiation (10.00) and number of roots per plantlets (12.00) when 1.0 mg IBA + 0.5 mg 2, 4-D/L was used. Maximum good results observed when 1.0 mg BAP was combined with different concentration of GA3 and KIN. Low concentration of IBA and 2, 4-D produces significant results. The findings of the present study could be useful to develop protocol to identify the potentiality of exact concentration of different growth regulators.
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